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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Mol Microbiol. 2003 Oct;50(2):549–561. doi: 10.1046/j.1365-2958.2003.03704.x

Fig. 5.

Fig. 5

A rpoS mutation reduces the cellular levels of Pol IV transcript. A. Ethidium stained agarose gel showing the dinB RT-PCR products from 250 ng of total endogenous RNA extracted from the wild type strain FC40 (upper band) and the RT-PCR products from the truncated dinB competitor RNA added to the same reactions at 1, 2, 4, 8, and 16 pg (lower band). M = 500 bp molecular weight marker. B. Ethidium stained agarose gel showing the dinB RT-PCR products from 250 ng of total endogenous RNA extracted from the rpoS::Cm strain PFG72 (upper band) and the RT-PCR products from the truncated dinB competitor RNA added to the same reactions at 0.5, 1, 2, 4, and 8 pg (lower band). M = 500 bp molecular weight marker. C. Plot and the least-squares fit of the log of the ratios of endogenous to competitor band intensities vs. the log of the amount of competitor RNA added. (To give a better fit, the 4 pg value for FC40, which appears to be an outlier, has been excluded; if it is included the line crosses y = 0 at x = 0.56 or 4.2 pg instead of at × = 0.69 or 4.9 pg).