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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Mol Microbiol. 2003 Oct;50(2):549–561. doi: 10.1046/j.1365-2958.2003.03704.x

Fig. 6.

Fig. 6

Pol IV levels increase in late stationary phase.

A. Western blot showing the amount of Pol IV protein in wild type (= FC40) and rpoS::Cm (= PFG72) cells at the time points indicated in B. Two blots are shown: on the left are samples taken during exponential growth through stationary phase; on the right are samples taken one and two days later. Samples c and d were reloaded for the blot on the right. Samples consisting of 40 μg of total protein were loaded (only half of the ΔdinB control lane on the left is visible).

B. The growth curve of the wild type (= FC40) and rpoS::Cm (= PFG72) mutant strain in minimal glycerol medium. Samples were taken at the times indicated by arrows.

C. The relative amounts of Pol IV protein in the wild type (= FC40) cells and rpoS::Cm (= PFG72) cells at the time points indicated in B. Bands on the two blots were normalized by first setting the most intense band for each strain on each blot equal; these were the band at d for the wild type strain, and the band at c for the rpoS::Cm strain. The intensity of each band was then recalculated relative to these. These values were then normalized to the wild-type strain sample a.