Fig. 7.
A rpoS mutation reduces adaptive mutation in various backgrounds. The accumulation of Lac+ mutations in Lac− cells incubated on lactose minimal medium. From left to right: Wild type = FC40; rpoS = PFG72; sulA = FC1417; sulA rpoS = PFG185; LexA(Ind−) = FC1418; LexA(Ind−) rpoS = PFG184; sulA LexA(Def) = FC1419, sulA LexA(Def) rpoS = PFG186; recG = FC526; recG rpoS = PFG36. Data are the mean number of Lac+ colonies appearing each day from days 3–5; each point is the mean of four cultures ± SEM. Three experiments are shown: the first was with the wild type and rpoS mutant strain; the second was with all the derivatives of the sulA mutant strain; and, for comparison, the data for recG and recG rpoS from the experiment shown in Fig. 3 were recalculated in the same format.