Figure 4.

Initial enzymatic rates of PET hydrolases

(A) Workflow for sampling PET reactions. Enzymes were added to reactions with PET, which were sampled over time. Samples were analyzed with HPLC, with monomers TPA, MHET, and BHET, and the sum of the three quantified.

(B–D) Quantified products of reactions up to 8 h, with 0.7 mg enzyme/g PET, 2.9% (w/v) PET coupons, at 70°C and pH 8, for benchmarks PHL7-WT (white circles), LCC-ICCG (black squares), and four engineered PHL7 variants (colored shapes). Points display the average of n = 3 reactions, while error bars display ±1 SD. (B) Concentration of total aromatic products. (C) Concentration of TPA. (D) Concentration of MHET.