Figure 5.

Depolymerization of amorphous PET film coupons as a function of time by PHL7 enzyme variants and LCC-ICCG

Plots show products of reactions up to 72 h, quantified with HPLC, with 0.35 mg enzyme/g PET, 2.9% (w/v) amorphous PET film coupons, at 70°C and pH 8, for benchmarks PHL7-WT (white circles), LCC-ICCG (black squares), and four engineered PHL7 variants (colored shapes). Points display the average of n = 3 reactions, while error bars display ±1 SD.

(A) Concentration of total aromatic products.

(B) Concentration of TPA.

(C) Concentration of MHET.