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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Biotechniques. 2005 Mar;38(3):413–424. doi: 10.2144/05383RV01

Figure 1. Different quantitative imaging approaches.

Figure 1

(A) Multicolor imaging to reveal protein co-localization can supply important information about the molecular composition of subnuclear domains. (B) The FRAP technique uses photobleaching of the labeled proteins within a ROI to measure the kinetics of the redistribution of the population of fluorescent-labeled proteins over space and time. (C) The analysis of subcellular structures using computer algorithms to automate the detection and measurement of subcellular features in large sets of high-resolution images. YFP, yellow fluorescent protein; NCoR, nuclear receptor corepressor protein; FRAP, fluorescence recovery after photobleaching; ROI, region of interest.