Skip to main content
. 2025 Aug 8;13:1630129. doi: 10.3389/fcell.2025.1630129

FIGURE 3.

Microscopic images and bar graphs illustrate the formation of vascular structures by DPSC and CD31/CD144 cells over time (2h, 4h, 24h). Panel a shows images; b-f show bar graphs of tube length, mesh, nodes, junctions, and segments, highlighting higher measurements in CD31&#+;/CD144&#+; cells compared to CD31-/CD144- cells, with significant differences at two hours.

CD31+/CD144+ positive cells exhibited greater tubule formation compared to CD31−/CD144− negative cells. Endothelial-like cells, sorted from DPSC-derived populations treated with HUVEC-ECM in the presence of DMP1, were seeded onto plates coated with growth factor-reduced Matrigel. Representative photomicrographs (a) show that the CD31+/CD144+ positive cell fraction formed more extensive tubule-like networks than the CD31−/CD144- negative fraction. Scale Bar = 250 μM. Measurements related to tubule formation in the negative (blue bars) and positive (green bars) fractions of the sorted cells include tube length (b), mesh area (c), number of nodes (d), junctions (e), and segments (f). Mean and SD from three measurements are presented. *: p < 0.05 and **: p < 0.01.