Figure 3.

Striatal levels of l-dopa and dopamine in completely lesioned animals injected with rAAV vectors. (A) Striatal TH enzyme activity was estimated 30 min after NSD-1015 by measuring the accumulation of l-dopa. Increased rAAV-mediated striatal l-dopa in the five-site 1:1 mix group was above the threshold value obtained with peripheral injection (dashed line). In contrast, animals receiving the TH vector alone or the 1:1 mixed vectors in only two sites did not produce this threshold l-dopa level. (B) Dopamine (DA) levels were reduced greatly on the lesioned side in all groups. Striatal dopamine was increased only in the 5 × 3-μl mixed-vector group.