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. 2025 Aug 8;16:1575106. doi: 10.3389/fphar.2025.1575106

FIGURE 2.

Panels A and B show tissue staining images: Hematoxylin and Eosin (H&E) and Oil Red O, respectively, displaying liver samples under different conditions—Control, MCD, MCD+H₂(L), and MCD+H₂(H). Panels C to O contain bar graphs comparing various biochemical and gene expression parameters across the same groups. Statistical significance is indicated by asterisks.

H2 therapy improved hepatic steatosis in mice fed with a MCD diet. (A) Liver H&E and (B) Oil Red O staining showed that both low and high doses of H2 attenuated MCD-induced hepatic steatosis. (C) Steatosis grade score, n = 8 mice in each group. (D) The levels of TG in the liver tissues, (E) Serum ALT levels, (F) Serum AST levels, n = 8 mice in each group. (G) The relative mRNA levels (ratios to Gapdh) of Acaca, (H) Fasn, (I) CD36, (J) Cpt1α, (K) Fabp1, (L) Acox, (M) Ppar-α, (N) Mttp, (O) Apob, n = 3 mice in each group. The data of steatosis grade score are expressed as median ± interquartile range, other results are expressed as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.