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. Author manuscript; available in PMC: 2005 Oct 15.
Published in final edited form as: J Biol Chem. 2005 May 20;280(28):26169–26176. doi: 10.1074/jbc.M502102200

Fig. 5. Summary of basal and hCG-stimulated cAMP levels in WT hLHR and mutants thereof expressed at the same cell surface densities.

Fig. 5

HEK293 cells were transiently transfected with increasing concentrations of cDNAs encoding the indicated hLHR constructs. In the same experiment, cell surface receptors (reflected by hCG binding assays to intact cells) and basal hCG-stimulated cAMP levels were determined as described under “Materials and Methods.” The cAMP values observed at 2.5 ng of hCG binding/106 cells were interpolated from the curves of each experiment. Within a given experiment, the hCG-stimulated cAMP value thus obtained from cells expressing WT hLHR was defined as 100%. The top panel shows the mean ± S.E. of data from at least three independent experiments for each construct and is expressed as the percent of hCG-stimulated cAMP in cells expressing the WT hLHR. In the bottom panel, the data from the top panel were used to express the hCG-stimulated cAMP as the -fold increase over basal.