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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Mol Microbiol. 2003 Aug;49(4):929–945. doi: 10.1046/j.1365-2958.2003.03619.x

Fig. 2.

Fig. 2

Detection of lig sequences in Leptospira species.

A. Southern blot analysis. Genomic DNA from L. interrogans Fiocruz L1-130 (middle lane) and L. biflexa Patoc 1 (right lane) were digested with NsiI and PacI and probed in Southern blots with ligA, ligB and ligC specific probes.

B and C. PCR analysis. Leptospiral DNA was subjected to PCR analysis by degenerate primers designed to anneal to all lig genes in L. kirschneri RM52 and L. interrogans Fiocruz L1-130. The expected size of the PCR product is −0.5 kb.