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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Mol Microbiol. 2003 Aug;49(4):929–945. doi: 10.1046/j.1365-2958.2003.03619.x

Fig. 3.

Fig. 3

Western blot analysis of LigA and LigB expression in low- and high-passage Leptospira.

A and B. Immunoblots of low (9 passages) and high-passage (>200 passages) isolates of L. kirschneri RM52 were probed with LipL41 antiserum (1:10 000), and either LigA antiserum (A, 1:2000) or LigB antiserum (B, 1:2000). L, low passage; H, high passage.

C and D. Immunoblots of L. kirschneri RM52 or L. interrogans Fiocruz L1-130 (passage 25 and passage 69) were probed with LipL41 (1:10 000) and LigA/B repeat antisera (1:5000).

E. An immunoblot of L. interrogans and L. kirschneri isolates was probed with LigA/B repeat antisera (1:5000) and GroEL antisera (1:30 000). Strain names are shown above each lane. P1, passage 1; HP, high passage. The relative mobility (kDa) of molecular mass standards (Bio-Rad) is shown on the left of each panel.