Table 1.

Plasmids used to express recombinant Lig proteins in E. coli.

Protein	Source	Tag	Amino acid co-ordinates	Plasmid name
LigA	RM52	His6	936–1224	p204orf1B
LigA	RM52	His6	342–1224	p204orf1
LigB	RM52	MBP	20–581	pROY1
LigB	RM52	MBP	579–1126	pROY2
LigB	RM52	MBP	296–847	pROY3
LigB	RM52	MBP	1113–1886	pJIM520
LigB	RM52	His6	1113–1886	pJIM518
LigC	RM52	His6	621–1060	p249orf1
LigB	L1-130	His6	131–649	pAE-ligBrep