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. 2002 Apr 2;99(7):4714–4719. doi: 10.1073/pnas.002031599

Figure 5.

Figure 5

S. cerevisiae rescue of phenotype. The S. cerevisiae heat shock sensitivity of cells lacking endogenous PDEs is rescued by a plasmid expressing TbPDE2B. JBS75 (PDE1 PDE2 containing p424), JBS67.2 (pde1 pde2 containing TbPDE2B on p424), and JBS67.1 (pde1 pde2 containing p424) were grown for 2 days at 30°C on selective plates, replica-plated to fresh selective plates, and held at 55°C or 30°C for 1 h before growing 2 days at 30°C. PDE represents cells that express PDEs; pde represents cells that do not express PDEs. Data are representative of three independent experiments.