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Intracellular PA and S1P were measured as described in the Experimental section. The histograms show the effect of (a) overexpression of LPP2 and LPP3 on basal intracellular PA and DG levels; and (b) overexpression of LPP2 and LPP3 on intracellular S1P produced from exogenous sphingosine and total [3H]sphingosine-labelled lipids. *P<0.05 compared with stable vector-transfected HEK-293 cells. V, vector.
