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. 2005 Sep 26;391(Pt 1):25–32. doi: 10.1042/BJ20050342

Figure 3. LPP2 and LPP3 attenuate p42/p44 MAPK activation via a caspase 3/7-dependent mechanism dependent on the apoptotic state of the cells.

Figure 3

Stable vector or LPP2- or LPP3-transfected HEK-293 cells were treated without or with Ac-DEVD-CHO (100 μM; 18 h) prior to stimulation with 1 μM LPA or 5 μM S1P for 10 min. (a) Western blot showing that Ac-DEVD-CHO blocks the ability of LPP2 and LPP3 to reduce the activation of p42/p44 MAPK by S1P and LPA respectively; (b) autoradiograph showing the cleavage of 35S-labelled PARP in lysates from vector and LPP2-overexpressing cells; (c) agarose gel showing the intranucleosomal DNA laddering in LPP2- and LPP3- overexpressing cells compared with vector-transfected cells; (d) protein determination (Bradford assay) in vector and LPP2- and LPP3-overexpressing cells after serum deprivation. Results are presented as the percentage of protein in vector-transfected cells before serum deprivation. *P<0.05 for vector-transfected versus LPP2- and LPP3-transfected cells (n=3 experiments).