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WT and RhBG-, RhCG- or RhAG-transfected HEK-293 cells were submitted to 32.5 mmol ammonium (A) or methylammonium (B) iso-osmotic inwardly-directed gradients (pH 7.0) at 15 °C. pHi changes were monitored through fluorescence change to the pH-sensitive dye BCECF in the stopped-flow spectrofluorimeter. A rapid initial alkalinization is observed. Smooth lines are exponential fits to the data.
