Table 1. Alkalinization rate constants (s−1) of transfected HEK-293 and MDCK cells.
Rate constant (s−1) | |||||||
---|---|---|---|---|---|---|---|
HEK-293 | MDCK | ||||||
Substrate | WT | RhBG | RhCG | RhAG | WT | RhBG | RhCG |
NH4+ | 0.18±0.011 (6) | 1±0.15* (5) | 0.88±0.07* (5) | 0.15±0.04 (3) | 0.18±0.009 (4) | 0.76±0.007* (4) | 0.45±0.02* (4) |
CH3NH3+ | 0.029±0.01 (6) | 0.53±0.06* (4) | 0.11±0.023‡ (3) | 0.04±0.01 (3) | 0.042±0.02 (4) | 0.35±0.01* (4) | 0.045±0.012† (4) |
C2H5NH3+ | 0.041 (2) | 0.11 (2) | 0.043 (2) | nd | nd | nd | nd |