Table 1. Secondary structure estimated by analysis of far-UV CD spectra of LytC and Cpl-1.
Secondary-structure content was estimated using CDNN and CONTIN (values in parentheses) methods from spectra recorded in the absence (−) and presence (+) of choline. Errors in the estimated values are 1–6% for CDNN and 1–4% for CONTIN. Values are rounded off to the nearest integer.
| Cpl-1 (%) | LytC (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| Structural element | − | + | − | + | ||||
| α-Helix | 11 | (7) | 8 | (4) | 12 | (8) | 7 | (12) |
| β-Sheet | 40 | (42) | 48 | (42) | 37 | (52) | 45 | (47) |
| Antiparallel | 35 | 42 | 31 | 40 | ||||
| Parallel | 5 | 6 | 6 | 5 | ||||
| β-Turn | 17 | (30) | 15 | (31) | 18 | (17) | 17 | (19) |
| Random structure | 32 | (21) | 29 | (23) | 33 | (23) | 31 | (22) |