Table 2. Band frequencies, fractional areas and proposed structure assignments of the components derived from IR measurements of LytC and Cpl-1.

Spectra were recorded in ²H₂O in the absence (−) or presence (+) of choline (10 mM phosphate, p²H 8.0). Values are rounded off to the nearest integer. Odd-numbered columns are values in cm⁻¹, even-numbered columns are percentages. ↑υ and ↓υ indicate high- and low-frequency components respectively.

LytC				Cpl-1
−		+		−		+		Assignment
1680	9	−	−	−	−	1683	1	β-Sheet (↑υ) and β-turns
−	−	1675	13	1677	11	1673	12	β-Sheet (↑υ) and β-turns
1668	13	1664	13	1659*	33	1660	18	β-Turns
1655	25	1650†	37	−	−	1650†	22	α-Helix, 310 helix and long loops
1640	32	−	−	1646	14	−	−	Unordered structure
−	−	1637	17	1637	24	1635	45	β-Sheet (↓υ)
1627	17	1628	17	1629	17	−	−	β-Sheet (↓υ)
1613‡	4	1612‡	3	1612‡	1	1614‡	2

* Maximum position could indicate some overlap with the α-helix component.

† Maximum position could indicate some overlap with the component from unordered structures.

‡ Bands at around 1612 cm⁻¹ in native proteins arise from amino acid side chains.