Table 3. Hydrodynamic parameters of LytC and Cpl-1.
Measurements were made in 20 mM phosphate buffer, pH 8.0, in the absence (Cpl-1 monomer) and in the presence of 60 mM (Cpl-1 dimer) and 140 mM choline (LytC monomer).
| LytC | Cpl-1 | ||
|---|---|---|---|
| Monomer | Monomer | Dimer | |
| s20,w (S) | 3.73±0.01 | 3.28±0.01 | 4.46±0.01 |
| sT20,w (S)* | 3.67±0.01 | 3.14±0.01 | − |
| f (g·s−1) | 6.83×10−8 | 5.47×10−8 | 7.82×10−8 |
| f/f0 | 1.41 | 1.27 | 1.44 |
| a/b (prolate)† | 4.6 | 2.9 | 5.0 |
| a/b (oblate)† | 5.0 | 3.1 | 5.5 |
* Calculated with HYDROPRO from the Cpl-1 structure and the 3D model of LytC.
† Axial ratio between length (a) and diameter (b) of the theoretical ellipsoids.