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(A) SDS/PAGE of molecular-mass markers (lane 1), Talon® metal-affinity-column-purified fusion protein A/C-e (lane 2); (B) Western blot of purified A/C-e fusion protein identified with anti-(atrolysin A) antibody. (C) Protein sequence of recombinant fusion protein A/C-e. Underlined is the amino acid sequence of the cysteine-rich domain of atrolysin A.
