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. 2005 Sep 26;391(Pt 1):69–76. doi: 10.1042/BJ20050483

Figure 2. Binding of atrolysin A, A/DC and A/C proteins to collagen I and to vWF by solid-phase binding assay.

Figure 2

Microtitre plates (96-well) were coated with 10 μg/ml rat skin collagen I (A–D) or vWF (E, F) followed by incubation with atrolysin A (A), A/DC (B, E) or A/C (C, F) proteins. (D) Competition of atrolysin A binding to immobilized collagen I by A/C protein. Collagen-I-coated wells were incubated with A/C protein, followed by incubation with 1.0 μg/well atrolysin A. Bound protein was detected as described in the Experimental section. Data are the means±S.D. of four determinations.