Table 1. Kinetic evaluation of the interaction of venom proteins with collagen I immobilized on CM-5 sensor chip in the BIAcore™ 3000 system.
Evaluation was performed according to a 1:1 binding model. Results are the means±S.D. for six different analyte concentrations. n.d., not determined.
| Kd (μM) | kd (×10−3 s−1) | ka (×103 M−1·s−1) | |
|---|---|---|---|
| Jararhagin | 0.0216 | 1.22±0.0084 | 56.4±1.3 |
| Atrolysin A | 0.117 | 4.71±0.0374 | 40.2±3.77 |
| Catrocollastatin C | 0.777 | 4.23±0.0258 | 5.45±0.105 |
| A/C-e | 0.0726 | 1.93±0.014 | 26.5±0.271 |
| Atrolysin C (Ht-C) | 509 | n.d. | n.d. |