Table 1. Purification of pGal3ST from porcine colonic mucosa.

Gal3ST activities were determined as described in the Experimental section.

Step	Total activity (nmol/min)	Protein (mg)	Specific activity (nmol/min per mg)	Yield (%)	Purification (fold)
Triton X-100 extract	903	3.40×104	26.6	100	1
First hydroxyapatite	173	1980	87.4	19	3.3
Second hydroxyapatite	76.0	778	97.7	8.4	3.7
Con A–Sepharose	19.4	11.2	1.73×103	2.1	65
Third hydroxyapatite	7.59	3.35	2.27×103	0.84	85
Mucin glycopeptide–Sepharose	4.45	0.31	1.44×104	0.49	540
Cu2+-chelating Sepharose	2.69	0.030	8.97×104	0.30	3370
AMP–agarose	1.29	0.003	1.29×106	0.14	48500