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The relative activity values were calculated with respect to the value of ‘None’ which was set as 100. The enzymatic activities represent the means for four independent experiments (S.D. was <5%). NEM, N-ethylmaleimide.
| Compound | Relative activity (%) |
|---|---|
| None | 100 |
| MnCl2 (10 mM) | 172 |
| CaCl2 (10 mM) | 116 |
| MgCl2 (10 mM) | 114 |
| EDTA (10 mM) | 89 |
| NEM (5 mM in 10 mM MnCl2) | 101 |
| DTT (5 mM in 10 mM MnCl2) | 63 |
