Table 3. Effects of various lipids and amines on pGal3ST activity.
Concentrations of lipids and amines were 0.005% (w/v). The relative activities were calculated with respect to the values of D-sphingosine, which was set as 100. The enzymatic activities represent the means for four independent experiments (S.D. was <5%).
| Compound | Relative activity (%) |
|---|---|
| L-α-Phosphatidic acid | <1 |
| α-Phosphatidylcholine | <1 |
| L-α-Lysophosphatidylcholine | <1 |
| L-α-Phosphatidylethanolamine | <1 |
| L-α-Phosphatidylinositol | <1 |
| L-α-Phosphatidyl-L-serine | <1 |
| Sphingomyelin | <1 |
| Ceramides | <1 |
| Spermine | 76 |
| Spermidine | 6 |
| Putrescine | 2 |
| D-sphingosine | 100 |
| N,N-Dimethylsphingosine | 86 |
| N-Acetyl-D-sphingosine | <1 |
| N-Stearoyl-D-sphingosine | <1 |
| Psychosine | 65 |
| Sphingosine 1-phosphate | 17 |
| Stearylamine | 103 |
| Octylamine | <1 |
| CHAPS | <1 |
| Hexadecyltrimethylammonium bromide | 105 |
| 1-Dodecylpyridinium chloride | 83 |