Table 4. Substrate specificity of pGal3ST.
The concentrations of the acceptors were 1 mM. The relative activities were calculated with respect to the value of core 1, which was set as 100. The enzymatic activities represent the means for four independent experiments (S.D. was <5%). GalNAc-OH, N-acetylgalactosaminitol.
| Acceptor | Relative activity (%) |
|---|---|
| Core 1 | 100 |
| Galβ1→3GalNAcα1-O-pNP | 68 |
| Galβ1→3GalNAcα1-O-Bn | 132 |
| Galβ1→3GalNAc-OH | <1 |
| Galβ1→3(GlcNAcβ1→6)GalNAcα1-O-pNP | 27 |
| Type 1 | 34 |
| Type 2 | 14 |
| Galβ1→4Glc | 8.5 |
| Galβ1→3GlcNAcβ1→3Galβ1→4Glc | 43 |
| Galβ1→4GlcNAcβ1→2Manα1→3/6Manβ1→4GlcNAc | 17 |
| BiGP | 15 |
| GalCer | <1 |
| LacCer | <1 |
| GalDG | <1 |