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The concentration of PAPS was 4 μM. The acceptor substrate for PAPS was 5 mM Galβ1→3GalNAc.
| Substrate | Km (mM) | Vmax (nmol/min per mg of protein) | Vmax/Km (nmol/min per mg of protein per mM) |
|---|---|---|---|
| Galβ1→3GalNAc | 3.7 | 57 | 15 |
| Galβ1→3GalNAcα1-O-pNP | 5.6 | 47 | 8.4 |
| Galβ1→3GlcNAc | 14 | 57 | 4.1 |
| Galβ1→4GlcNAc | 30 | 51 | 1.7 |
| PAPS | 0.026 | 217 |
