Table 6. Substrate competition experiments with pGal3ST.
| Total velocity (nmol/min per mg of protein) | ||||
|---|---|---|---|---|
| Expected | ||||
| Substrates | Observed | One enzyme | Two enzymes | Suggested number of enzymes |
| Type 1 (10 mM) | 23.7±1.3 | |||
| Type 2 (10 mM) | 13.9±0.7 | |||
| Core 1 (3 mM) | 26.3±0.6 | |||
| Type 1 (10 mM)+core 1 (3 mM) | 33.5±1.1 | 34.5 | 50.0 | 1 |
| Type 2 (10 mM)+core 1 (3 mM) | 30.5±0.5 | 29.5 | 40.2 | 1 |