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(A) TLC separation of α32P GDP and GTP in assays with Rab14 alone, Rab14 plus the GAP domain, and Rab14 plus the R/K mutant of the GAP domain. (B) The data from (A) are presented as the percentage of the total radioactivity in GDP versus time for Rab14 (●), Rab14 plus the GAP domain (■), and Rab14 plus the R/K mutant GAP domain (▲). For further details, see the Experimental section.
