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. 2005 Sep 26;391(Pt 1):87–93. doi: 10.1042/BJ20050887

Figure 4. Rabs in GLUT4 vesicles.

Figure 4

GLUT4 vesicles (G4V) and control adsorbates (CV) were prepared from unstimulated (B) and insulin-treated (I) 3T3-L1 adipocytes, as described in the Experimental section. SDS samples of the vesicles were immunoblotted for IRAP, Rabs 2, 8 and 14, and AS160. The 1× loads were derived from 0.15% of a 10-cm diameter plate for IRAP, 2% of a plate for Rabs 2, 8 and 14, and 4% of a plate for AS160. Two repetitions of this experiment gave similar results, except for variable relative amounts of Rab14 in the insulin-treated GLUT4 vesicles (see the text).