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. 2005 Sep 26;391(Pt 1):105–114. doi: 10.1042/BJ20050328

Figure 2. LdPEX14–LdPEX5 interaction.

Figure 2

The interaction of His6/S–LdPEX14 (A) or His6/S–ldpex14-(1–120) (His6/S–ldpex14-120) (B) with either the wild-type LdPEX5 or ldpex5 WXXXY/F site-directed mutants was assessed by pull-down assays using S-protein agarose beads. LdPEX14 proteins were mixed with 10 μg of purified, ldpex5-W53A, ldpex5-W293A, ldpex5-W176,293A, ldpex5-W53,176,293A or wild-type LdPEX5 or with no LdPEX5 and the mixture was added to S-protein agarose. Beads were stringently washed with 1% Triton X-100 and 500 mM NaCl in TBS (Tris-buffered saline; 50 mM Tris/HCl, pH 8.0 and 150 mM NaCl) and bound proteins were analysed by Coomassie Blue-stained SDS/PAGE.