Table 2. Equilibrium dissociation constants for the ldpex5–LdPEX14 interaction.

Constructs correspond to the schematic diagram in Figure 4(A). Numbers in parentheses indicate the number of times the experiment was performed.

LdPEX5	LdPEX5 construct	Kd (nM)
ldpex5-(203–391)	3	192±31 (2)
ldpex5-(290–391)	4	116±34 (1)
ldpex5-(203–269)	5	No binding (2)
ldpex5-Δ181–313	7	69±16 (4)
ldpex5-(1–270)	8	No binding (3)
ldpex5-(283–625)	10	111±57 (1)
ldpex5-(1–202)	12	No binding (4)
ldpex5-(268–303)	13	No binding (2)