Figure 2. Stability of N-chloro derivatives of glycosaminoglycans and glycosamine monosaccharides on incubation at 37 °C in pH 7.4 buffer.

Time-dependent changes in the concentrations of N-chloro derivatives were determined by assay with TNB and expressed as percentage of the initial value. Monosaccharide and polymer N-chloro derivatives were prepared as described in the text. The preparation of polymer dichloramines was initially assayed 0.5 min after the addition of HOCl, i.e. before complete HOCl consumption (see text). Data for HA chloramides is reproduced from [22]. (A) Monosaccharide N-chloro derivatives: GlcNH₂ dichloramines (●); GlcNH₂ chloramines (△); GlcNSO₃ N-chlorosulphonamides (grey diamonds); GlcNAc chloramides (▼). (B) Polymer N-chloro derivatives: p-HS dichloramines (●), dnsp-HP dichloramines (○), dnsp-HP chloramines (△), p-HP NCl (grey diamonds), b-HP NCl (◇) and HA chloramides (▼).