Figure 4. Dichloramine-dependent fragmentation of glycosaminoglycans at 37 °C and pH 7.4.

Densitometric traces of polymer samples analysed by PAGE with Alcian Blue staining. (A) dnsp-HP (6.4 mg·ml⁻¹; 1.815 mM GlcNH₂ residues) reacted with HOCl (3.63 mM) at 37 °C for 0 (–––), 6 (····), and 24 h (———). (B) p-HS (6.4 mg·ml⁻¹; 0.225 mM GlcNH₂ residues) reacted with HOCl (0.45 mM) at 37 °C for 0 (–––) and 24 h (———). Grey arrowhead, migration position of Bromophenol Blue; black arrowhead, migration position of heparin octasaccharide standards.