Table 1. Residue abundance/substitution data for glycosaminoglycans (expressed per 100 disaccharides).
p-HS* | dnsp-HP* | p-HP† | b-HP† | HA | |
---|---|---|---|---|---|
GlcNH2 | 1.7 | 12.5 | 0 | 0 | 0 |
GlcNSO3 | 43.4 | 76.1 | 88.6 | 97.7 | 0 |
GlcNAc | 55.0 | 11.4 | 11.4 | 2.3 | 100 |
2-O-sulphate | 19.9 | 75.6 | 75.6 | 89.3 | 0 |
6-O-sulphate | 21.9 | 81.5 | 81.5 | 92.4 | 0 |
Average molecular mass of disaccharide (Da)‡ | 470 | 607 | 615 | 645 | 401 |
* Data from [25], except for abundance of GlcNH2, which was determined as described in the text; an additional preparation of dnsp-HP used was determined to have the following glycosamine residue abundances (residues per 100 disaccharides): GlcNH2, 17.1; GlcNSO3, 71.5; and GlcNAc, 11.4 (average molecular mass of disaccharide 604 Da).
† Data from [25].
‡ Calculated with respect to dry weight on the basis of the abundance/substitution data; carboxylate and sulphate groups were assumed to have sodium (Na+) counter-ions, and free amine groups were assumed to exist as hydrochloride (HCl) salts.