Table 1. Partial-proteolysis fragments of (His)₈-rP[I-V].

The protein bands (partial-proteolysis fragments) labelled alphabetically in Figure 3(A) were subjected to N-terminal sequencing, the results of which were used to decide their N-terminal positions. The molecular masses were calculated from the migration on SDS/PAGE, which was performed several times to yield errors less than 0.5 kDa. These data were used for prediction of the C-terminal positions. When the fragments seemed to be produced by cleavage at their N-terminal ends only, the ‘position of C-terminal aa’ is indicated as 520.

Fragment	Protease	N-terminal sequence	Position of N-teminal aa	Predicted molecular mass (kDa)	Predicted position of C-teminal aa
a	Trypsin	TTEGL	60	52	520
b	Trypsin	LSSYP	69	50	515
c	Trypsin	RANKK	85	47	501 or 502 or 503
d	Trypsin	RAANS	92	46	501 or 502 or 503
e	Glu-C	VASTK	35	55	520
f	Glu-C	VASTK	35	52	495 or 498
g	Glu-C	GLPNM	63	49	484
h	Glu-C	VASTK	35	47	451 or 454
i	Glu-C	GLPNM	63	44	451 or 454
j	Glu-C	GLPNM	63	43	451 or 454
k	Lys-C	NINSP	50	53	520
l	Lys-C	LSSYP	69	50	520
m	Lys-C	NINSP	50	46	464
n	Lys-C	LSSYP	69	43	455
o	Lys-C	GRRAA	90	41	455