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. 2005 Sep 26;391(Pt 1):143–151. doi: 10.1042/BJ20050229

Figure 3. Specificities of Gab1 and Gab2 siRNAs.

Figure 3

(A) Alignment of human Gab1 and Gab2 siRNA targeting nucleotide sequences with the corresponding sequences in mouse Gab1 and Gab2. (B, C) COS-7 cells were co-transfected with pGab1siRNA, pGab2siRNA or empty vector (1.8 μg) and FLAG-tagged human Gab1 (hGab1), human Gab2 (hGab2), mouse Gab1 (mGab1) or mouse Gab2 (mGab2; 0.2 μg) as indicated. Cell lysates (30 μg) were subjected to immunoblot analyses with antibodies to the FLAG-tag and β-actin after 48 h transfection.