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. 2005 Sep 26;391(Pt 1):143–151. doi: 10.1042/BJ20050229

Figure 7. Effects of adenoviral-based siRNAs on endogenous Gab protein levels and Mek1 activity.

Figure 7

T47D cells were incubated with recombinant adenoviruses as indicated for 16 h and then with fresh medium for 48 h followed by serum starvation and EGF stimulation. AdGab1+2siRNA indicates a 1:1 mixture of AdGab1siRNA and AdGab2siRNA viruses (MOI 125). Aliquots of cell lysates were analysed by immunoblotting with antibodies to Gab1, Gab2 and β-actin (A). Mek1 was immunoprecipitated from cell lysates and Mek1 kinase activity was determined (B). The histogram represents data from two independent experiments performed in duplicates (n=4).