2.

Semipreparative HPLC-PDA microfractionation of the ethyl acetate extract of K. oblongifolia leaves (30 min chromatographic). Vertical lines correspond to the 92 microfractions that were tested for their anti-infective activities in infection (in red), their effect on the host in infection (in green) as well as antibiotic activities in broth (in purple) with 3 biological replicates, and 1 technical replicate for each experiment. Ten zones (hit microfractions) with distinct m/z values detected and with inhibitory activities above 50% can be observed. Two more microfractions with distinct m/z values and inhibitory activities close to 50% (but not above) were also considered hits (M08 and M31). The 30 min chromatogram was truncated between 0 and 21 min to focus on bioactive regions.