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. 2025 Aug 8;5(4):738–752. doi: 10.1021/acsbiomedchemau.5c00091

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Experimental validation of the predicted mutagenesis effect on trans-splicing efficiency in combination of SspN-GFP and versions of SspC-GFP (short name on plot SSP N+C): (A) fluorescence microphotographs of 48 h post-transfected HEK293 cells captured by the IncuCyte S3 with parameters: six images per well with 10× magnification and 300 ms of GFP exposure. (B) Flow cytometry analysis of GFP+ cells population, (C) flow cytometry analysis of MFI values normalized to the fluorescence of the Ssp N+C sample in 10-base logarithmic scale, (D) enlarged version of MFI values normalized to the fluorescence of the Ssp N+C sample, (E) kinetic curves of green objects per image accumulation from cells during 24 h incubation in IncuCyte S3. (*) p-value <0.05, (**) p-value <0.01, (***) p-value <0.001, and­(****) p-value <0.0001.