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. 2025 Aug 8;15:1608664. doi: 10.3389/fonc.2025.1608664

Table 4.

Clinicopathological and molecular characteristics of G1, G2, and G3 in this study.

Clinicopathologic characteristics Number of patients (N=3937) G1: RNF43 codon 659-mutated, Non-class1 BRAF-mutated, and MSI-H, N (%) (N=34) G2: Not G1 and G3, N (%) (N=3816) G3: RNF43 Non-codon 659-mutated, Class 1 BRAF-mutated, and Non-MSI-H, N (%) (N=87) P value (G1 vs. G2) P value (G1 vs. G3) P value (G2 vs. G3)
Age <0.0001 <0.0001 0.189
Young (years <50) 1300 (33.02%) 1 (2.94%) 1261 (33.05%) 38 (43.68%)
Intermediate (<70 years ≥50) 1647 (41.83%) 11 (32.35%) 1605 (42.06%) 31 (35.63%)
Elder (years≥70) 963 (24.46%) 22 (64.71%) 923 (24.19%) 18 (20.69%)
NA 27 (0.69%) 0 (0.00%) 27 (0.71%) 0 (0.00%)
Gender 0.1876 0.2404 0.0647
Female 1845 (46.86%) 21 (61.76%) 1782 (46.70%) 42 (48.28%)
Male 2030 (51.56%) 13 (38.24%) 1976 (51.78%) 41 (47.13%)
NA 62 (1.57%) 0 (0.00%) 58 (1.52%) 4 (4.60%)
Primary tumor location <0.0001 <0.0001 0.541
Right 916 (23.27%) 30 (88.24%) 867 (22.72%) 19 (21.84%)
Left 2090 (53.09%) 1 (2.94%) 2046 (53.62%) 43 (49.43%)
NA 931 (23.65%) 3 (8.82%) 903 (23.66%) 25 (28.74%)
TNM stage <0.0001 0.0087 0.0855
I 425 (10.80%) 4 (11.76%) 406 (10.64%) 15 (17.24%)
II 743 (18.87%) 18 (52.94%) 704 (18.45%) 21 (24.14%)
III 1224 (31.09%) 10 (29.41%) 1190 (31.18%) 24 (27.59%)
IV 1375 (34.93%) 1 (2.94%) 1352 (35.43%) 22 (25.29%)
NA 170 (4.32%) 1 (2.94%) 164 (4.30%) 5 (5.75%)
TUMOR_GRADE <0.0001 0.0767 0.152
Well differentiated 509 (12.93%) 4 (11.76%) 495 (12.97%) 10 (11.49%)
Moderately differentiated 1351 (34.32%) 6 (17.65%) 1318 (34.54%) 27 (31.03%)
Moderate poorly differentiated 110 (2.79%) 1 (2.94%) 105 (2.75%) 4 (4.60%)
Poorly differentiated 333 (8.46%) 13 (38.24%) 307 (8.05%) 13 (14.94%)
NA 1634 (41.50%) 10 (29.41%) 1591 (41.69%) 33 (37.93%)
TMB <0.0001 <0.0001 <0.0001
TMB-H 858 (21.79%) 34 (100.00%) 789 (20.68%) 35 (40.23%)
TMB-L 2745 (69.72%) 0 (0.00%) 2700 (70.75%) 45 (51.72%)
NA 334 (8.48%) 0 (0.00%) 327 (8.57%) 7 (8.05%)
MSI <0.0001 <0.0001 0.898
MSI-H 34 (0.86%) 34 (100.00%) 0 (0.00%) 0 (0.00%)
MSS 2834 (71.98%) 0 (0.00%) 2768 (72.54%) 66 (75.86%)
NA 874 (22.20%) 8 (23.53%) 845 (22.14%) 21 (24.14%)
BRAF status <0.0001 <0.0001 >0.9999
BRAF mut 329 (8.36%) 34 (100.00%) 289 (7.57%) 6 (6.90%)
BRAF wild-type 3608 (91.64%) 0 (0.00%) 3527 (92.43%) 81 (93.10%)
BRAF mutation types 0.0008 <0.0001 <0.0001
Class 1 224 (5.69%) 34 (100.00%) 190 (4.98%) 0 (0.00%)
Class 2 9 (0.23%) 0 (0.00%) 9 (0.24%) 0 (0.00%)
Class 3 40 (1.02%) 0 (0.00%) 40 (1.05%) 0 (0.00%)
NA 56 (1.42%) 0 (0.00%) 50 (1.31%) 6 (6.90%)