2.

(a) Schematic of the flow channel in order illustrate the rectangular shape, key dimensions (including the reservoir gap), and the flow direction. (b) COMSOL simulation with a 0.5 mL/min volumetric flow rate showing the fluid flow rate profile along the length of the channel as a 2D slice. Note that there is a monolayer of cells (50 × 50 array) at the center bottom wall of the channel in (b). (c) Overlaid results of variance (from flow injection experiments) and the inverse of estimated shear stress (from COMSOL simulations) as a function of the volumetric flow rate.