2.

Concentrations of glucose for n = 4 donors across 56 days of storage in AS-1N is shown in (A). Of the 68 measurements shown, 48/64 (75%) are within the range of 5 ± 1 mM. The glucose levels were maintained by measuring the glucose concentration of an aliquot removed from the storage bag, followed by the addition of an appropriate volume of a sterile glucose stock solution using the system described in Figure . The percentage of lysed RBCs within the storage bag is shown in (B). Importantly, the lysis rate for the AS1-N stored RBCs remained under 1% (required by the American Association of Blood Bankers, AABB) up through 6 weeks of storage; however, both storage methods resulted in lysis rates above 1% beyond 6 weeks of storage. For both measurements, n = 4 donors, and error bars represent SEM.