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. 2025 Apr 3;5(4):469–476. doi: 10.1021/acsmeasuresciau.5c00001

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Raw AFM-IR maps of EVs taken at (a) 1392 cm–1, (b) 1620 cm–1, (c) 1646 cm–1, and (d) 1737 cm–1. The images show high location dependence of the AFM-IR signal within the EV, marked by the arrows, and large differences in distribution at different wavenumbers. The corresponding topography image is found in Figure b.