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. 2002 Mar 26;99(7):4730–4735. doi: 10.1073/pnas.072626099

Figure 6.

Figure 6

Southern blot and RT-PCR of KNAT1. (A) Southern blot. Genomic DNA from Col wt (lanes 1 and 2), Ler wt (lanes 3 and 4), RLD wt (lanes 5 and 6), bp-1 Ler (lanes 7 and 8), and bp-2 RLD (lanes 9 and 10) was digested with BamHI (lanes 1, 3, 5, 7, and 9) or EcoRI (lanes 2, 4, 6, 8, and 10) and probed with the KNAT1 cDNA. Sizes of the molecular weight standards (kb) are indicated. (B) RT-PCR using KNAT1 primers 954 and 955. Lane 1, Col wt; lane 2, RLD wt; lane 3, Ler wt; lane 4, bp-1 Ler; lane 5, bp-2 Ler; lane 6, bp-2 RLD; lane 7, bp-2 Col. The same cDNA pools were amplified with primers specific for glyceraldehyde-3-phosphate dehydrogenase (gapC; ref. 36).