Figure 2.

(A) Northern blot analysis of GCR1 mRNA abundance. Each lane contains 10 μg of RNA from six different BY-2-transformed lines (1–6). GCR1-coding sequence was used as probe. (B) GTPγ³⁵S-binding assay. Receptor activity was measured in membrane preparations by determining the binding of the nonhydrolyzable GTP analog GTPγ³⁵S. Binding of GTPγ³⁵S in Arabidopsis T2 plants overexpressing GCR1 (hatched bar) was significantly higher compared to the wt Arabidopsis plant (open bar). Error bars indicate SD.