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. Author manuscript; available in PMC: 2026 Jul 13.
Published in final edited form as: Macromol Biosci. 2025 Jul 13;25(11):e00636. doi: 10.1002/mabi.202400636

Figure 2. Effects of Store-Operated Calcium Channel Inhibition on MC-GAG-mediated Osteogenic Differentiation and Mineralization.

Figure 2.

(A) Schematic of chemical inhibition of Orai1 with N-propargylnifrendipine (MRS1845). (B) WST-8 assay of hMSCs cultured for 7 days in the presence of growth media with or without MRS1845. (C) qRT-PCR for alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN) expression in primary hMSCs cultured on either Col-GAG or MC-GAG for 7 days in the presence of OGM alone or with the addition of MRS1845 (n=3). (D) Representative images of Alizarin red staining and (E) quantitative analysis for empty Col-GAG or MC-GAG scaffolds (Empty) or hMSCs cultured for 8 weeks in the presence or absence of MRS1845 on respective scaffolds. (F) Representative images and (G) densitometric quantification of Western blots for phosphorylated ERK1/2 (p-ERK1/2), total ERK1/2, phosphorylated Smad1/5 (p-Smad1/5), total Smad5, Runx2, and GAPDH in hMSCs cultured on Col-GAG or MC-GAG for 7 days with or without MRS1845. Bars represent means, errors bars represent standard error. Asterisks denote significant comparisons. *, p<0.05; **, p<0.01; ***, p<0.001.