Skip to main content
. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Mol Microbiol. 2024 Jun 18;122(1):113–128. doi: 10.1111/mmi.15286

Figure 2. Thiourea does not protect cells from the superoxide that is generated by a redox-cycling antibiotic.

Figure 2.

Cells growing in aerobic glucose medium were treated with paraquat (PQ) starting at t=0. All cells (MG1655, KCI746) were SodB+; where indicated, strains lacked the inducible SodA superoxide dismutase, in order to establish that the growth defect arose from superoxide. (A) Thiourea (TU, 150 mM) was supplemented as indicated. (B) Parallel sodA cultures were supplemented with 10 mM glutathione (GSH), 10 mM N-acetylcysteine (NAC), 0.1 mM edaravone (Eda), 10 mM Tiron, or 5 mM ascorbate (Asc).